How many times can you use a SCOBY?
Time to switch up your SCOBY for a flavor boost in your brew.

When to replace your SCOBY?
A question that may come up in your brewing journey is: “Do I ever need to replace my SCOBY? If so, when?” This question operates under the assumption that we’re referring to just the pellicle (rubbery surface growth) as the SCOBY. If you’re wondering what’s the difference between the pellicle and the SCOBY, take a look at our post here – What is a SCOBY. The SCOBY is more than just the pellicle, which I’ll go more in detail about later, but I wanted to clarify that this question is derived from confusion on the definition of a SCOBY. The direct answer is that you never need to replace your SCOBY if well taken care of, but when it comes to the pellicles (or “mothers” as they are so lovingly referred to), those can be thinned out with each brew.
Separating the SCOBY, mentally and physically
Mentally: SCOBY is an obscure term and has, over time, ubiquitously come to refer to the rubbery, pancake looking surface growth that occurs on your kombucha brew. This is somewhat of a misrepresentation because the acronym SCOBY stands for Symbiotic Culture Of Bacteria & Yeast–referring to the living microbial population residing in the kombucha. The pellicle is made up of cellulose, synthesized by the bacteria during fermentation i.e., not bacteria or yeast, and not alive. There are definitely microbes living in and around the cellulose structure, but the pellicle is more like their little house (quite the mansion, actually). It is hard to think of yeast and bacteria on their own without some sort of substrate–which is why I suspect the pellicle has become so esteemed in the kombucha brewing world. It’s a bit more accurate to think of the starter tea itself as the SCOBY, though I suppose the same argument could be used against that statement as well. The point of me explaining this is to separate the idea of the cellulose mat being the conductor of fermentation. When it is merely a by-product of the fermentation, which occurs regardless of if there were any previous introduction of the substance.
Physically: Like I was saying, the fermentation of kombucha produces a cellulose mat that gets synthesized on the surface of the tea. This mat will get thicker with time if sat undisturbed. If disturbed, a layer of liquid can get on top, and a new layer will begin to form on the surface again. Each new brew causes surface disturbance, and thus new layers will likely form with each brew. Eventually, your brewing vessel can become overrun with pellicles, and you will be asking yourself what the heck to do with all these pellicles! As far as the kombucha itself is concerned, you can toss the pellicles with each brew. That’s not to say that the pellicle is useless, however, because microbial cellulose is a remarkable material that can be fashioned into all kinds of crazy things (fruit leathers, dog treats, jewelry, clothing, etc.!).

Layered Kombucha Pellicles
Can a SCOBY go bad?
Often people will create a SCOBY hotel and allow previously grown pellicles to preserve in kombucha, being kept for backup use in case something goes awry with their main brew. While we have talked about how adding a pellicle isn’t required for the fermentation to take place, it is certainly not hurting anything and only adds extra microbes that are living on the surface of the pellicle. So while creating a SCOBY hotel might not be necessary (an alternative would be just to have a spare jar of starter tea), there is some viability to the practice and allows a “waiting room” for the pellicles when you don’t really know what to do with them.
So in a SCOBY hotel, can the pellicles go bad? As long as there is enough liquid to keep the pellicles covered, they will preserve for years in my experience. The more common problem here is allowing the liquid to evaporate, or going too long without checking on it which allows the top pellicle to dry out and will inevitably mold.
When is it time to replace your SCOBY?
Here I’m referring to the SCOBY in its entirety, as in, when do you need to start over completely? You will only need to replace your SCOBY if it has developed mold or if it is continuously struggling to ferment. Often with struggling SCOBYs, things can be done to help bring balance to your culture depending on the situation, and you won’t need to start over completely. If you aren’t getting good results, a quicker fix sometimes is to start fresh with a new, strong culture so you can enjoy your homebrewed kombucha again.
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Continue Reading
- What’s The Brown Stuff in Kombucha
- Continuous Brew Kombucha: Step by Step Instructions
- What is a SCOBY?
- Mason Jar Fermentation Kit
- How to Make SCOBY Fruit Leathers
- Learn The Health Benefits of Kombucha
- Brew With Our Kombucha Kit
Other Ferments
- How to Make Fermented Ketchup
- How to Make Zesty Italian Dressing Recipe
- Try Our Delicious Kosher Dill Pickle Recipe
- Easin’ Into Cheesin’: Classic Burrata Recipes
- Easy Beer Cheese Dip Recipe
I. Had. No. Idea!!!!!! I had ~8 pellicles in my fridge previously and so eventually threw them away and just recently purchased another starter from you. My first batch came out perfectly, mmmmm! But since I had so many pellicles sitting around previously, I decided not to keep the mother and use the new one!!!! I am hoping I didn’t make a huge mistake!!!
lol no You didn’t mess anything up but actually did what I would do and get rid of extras so they’re not just piling up on you. You can use the new one, no worries!
I have used my scoby to brew two batches of kombucha. Neither batch had any pellicles form but they fermented fine and tasted great. I did have stringy things form hanging off the scoby and I did have a layer of sediment form on the bottom of my brewing jar.
Is it unusual that no pellicles form?
Thanks!
John
Hi John,
It’s not totally unusual. Great kombucha can be produced with no pellicle formation.
To get pellicle formation, you can do two things:
1. In your main brewing vessel, increase the dissolved oxygen by stirring the brew vigorously in the first few days.
2. In a separate jar, take at least 4 cups of your brewed kombucha, cover with a breathable cover and let it ferment alone in a jar for a while. After a few weeks you can expect some pellicle formation on there. Then you can add that strong liquid to your brewing jar with your starter tea to get a really strong brew.
Hello! I have been brewing kombucha now for a few months and had some great results, however, recently when I have been bottling my kombucha I have found that I am getting spotting on my pellicle in the bottles only, the large pellicle looks fine. Is this a sign that I need to start over or is it possible that it is just the juice that I have started bottling with. I have also noticed that I am not getting the carbonation build up like I was before.
Any guidance is appreciated,
Kelsey
Hi Kelsey! You shouldn’t have pellicle formation in the bottles. If you do, this is a usually a sign that your bottles aren’t air tight, which would also lead to a loss of carbonation. What type of bottles are you using?
What can you do to bring the Scoby back into balance? I have used mine tons of times to brew and it is always way too sweet tasting even though I let it sit for two weeks or more sometimes. I figures it wasn’t fermenting properly and eating the sugar but i am not sure why. Thank you for any help youcan give.
Bex!
Hi Bex! If it is tasting sweet still after two weeks, I would recommend switching up the type of tea and brewing a stronger batch, increase the temperature if possible to close to 78°F and decrease the amount of sugar you’re starting with. This will provide a nutrient-rich environment for the yeast, in their comfort zone, which should increase the odds of them getting to their most active state.
Can the left over initial brew, the brew that grew the scoby, the vinigery tea.Can it be consumed? does it have any health benefit? can it be used to start a new scoby growth. I took a sip, tastes like ACV.
Thanks, great site
Hi John and thanks for reaching out. When the brew is finished, the brew in its entirety is good to consume. The reason we save starter tea is to brew that next batch. As far as health benefits, please check out our article below:
The Health Benefits of Kombucha
Hope this helps and let us know if we can be of further assistance!